Efficient Production of Murine Uterine Damage Model
10.1007/s13770-018-0149-3
- Author:
Yoon Young KIM
1
;
Bo Bin CHOI
;
Ji Won LIM
;
Yong Jin KIM
;
Sung Yob KIM
;
Seung Yup KU
Author Information
1. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea. jyhsyk@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Uterine damage;
Endometrium thickness;
Murine model;
Embryonic implantation
- MeSH:
Anesthesia;
Animals;
Copper;
Dilatation;
Endometrium;
Ethanol;
Female;
Gynecology;
Horns;
Humans;
Infertility;
Isoflurane;
Mice;
Models, Animal;
Primates;
Regeneration;
Reproductive Medicine;
Uterus
- From:
Tissue Engineering and Regenerative Medicine
2019;16(2):119-129
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Thin or damaged endometrium causes uterine factor-derived infertility resulting in a failure of embryonic implantation. Regeneration of endometrium is a major issue in gynecology and reproductive medicine. Various types of cells and scaffolds were studied to establish an effective therapeutic strategy. For this type of investigations, production of optimal animal models is indispensable. In this study, we tried to establish various murine uterine damage models and compared their features. METHODS: Three to ten-week-old C57BL/6 female mice were anesthetized using isoflurane. Chemical and mechanical methods using ethanol (EtOH) at 70 or 100% and copper scraper were compared to determine the most efficient condition. Damage of uterine tissue was induced either by vaginal or dorsal surgical approach. After 7-10 days, gross and microscopic morphology, safety and efficiency were compared among the groups. RESULTS: Both chemical and mechanical methods resulted in thinner endometrium and reduced number of glands. Gross morphology assessment revealed that the damaged regions of uteri showed various shapes including shrinkage or cystic dilatation of uterine horns. The duration of anesthesia significantly affected recovery after procedure. Uterine damage was most effectively induced by dorsal approach using 100% EtOH treatment compared to mechanical methods. CONCLUSION: Taken together, murine uterine damage models were most successfully established by chemical treatment. This production protocols could be applied further to larger animals such as non-human primate.
