Effects of RetroMTA on osteoblastic differentiation in MC3T3-E1 cells
10.14815/kjdm.2018.45.2.97
- Author:
Hyo Il LEE
1
;
Sung Hyeon CHOI
;
Ji Hyun JANG
;
Hoon Sang CHANG
;
Yun Chan HWANG
;
In Nam HWANG
;
Bin Na LEE
;
Won Mann OH
Author Information
1. Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea. bnlee13@jnu.ac.kr, wmoh@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
MTA;
RetroMTA;
osteogenic differentiation;
mineralization
- MeSH:
Cell Survival;
Dentin;
Gene Expression;
Miners;
Osteoblasts;
Pemetrexed;
Real-Time Polymerase Chain Reaction
- From:
Korean Journal of Dental Materials
2018;45(2):97-110
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mineral trioxide aggregate (MTA) provides a very good sealing, acceptable biocompatibility, dentin bridge formation and osteo-inductive effect. However, MTA has a few drawbacks. RetroMTA including calcium-zirconia complex has been developed to overcome drawbacks of MTA, especially long setting time and difficult handling characteristics. Therefore, the aim of this study was to evaluate the effects of RetroMTA on biocompatibility, osteogenic differentiation and mineralization in MC3T3-E1 cells compared with conventional MTA. Cytotoxicity was assessed by WST-1 assay. The gene expression of osteogenic markers was detected by real-time PCR. ALP activity and mineralization behavior were evaluated using ALP and alizarin red staining. There was no statistically significant difference between ProRoot MTA and RetroMTA with material extracts at dilutions of 1/4, 1/10 and 1/50 in cell viability assay. Expression of osteogenic markers in both ProRoot MTA and RetroMTA groups was significantly higher than in control group. In the mineralization assay, RetroMTA showed significantly higher formation of mineralized nodules than in control. In conclusion, RetroMTA promotes osteogenic differentiation and potential mineralization. It appears to be a potential alternative to ProRoot MTA as a root-end filling material.
