Content Determination of Four Index Components in Compound Baizhi Zhitong Capsules by QAMS Method
10.3969/j.issn.1005-5304.2018.09.021
- VernacularTitle:一测多评法测定复方白芷止痛胶囊中4种指标成分
- Author:
Guang-Wei HUANG
1
;
Su-De YANG
;
Jing-Di CAO
Author Information
1. 上海绿谷生命园医药有限公司
- Keywords:
Compound Baizhi Zhitong Capsules;
quantitative analysis of multi-components by single-marker;
chlorogenic acid;
ferulic acid;
imperatorin;
isoimperatorin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(9):86-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method for content determination of chlorogenic acid, ferulic acid, and isoimperatorin in Compound Baizhi Zhitong Capsules through quantitative analysis of multi-components by single-marker (QAMS) method by setting imperatorin as internal standard substance. Methods HPLC was adopted. The chromatographic column of Thermo Hypersil GOLD (4.6 mm×250 mm, 5 μm) was used; the mobile phase was composed of acetonitrile and 0.2％ formic acid in gradient elution; the flow speed was 1 m L/min; the detection wavelength were 320 nm (chlorogenic acid, ferulic acid) and 300 nm (imperatorin and isoimperatorin). The relative analysis correction factors of imperatorin to other three components were established by setting imperatorin as internal standard substance. Results The relative analysis correction factors of chlorogenic acid, ferulic acid, and isoimperatorin in Compound Baizhi Zhitong Capsules were 1.16, 2.00, and 1.00, which had a high reproducibility. There was no significant difference between QAMS and external standard method. Conclusion The QAMS method for Compound Baizhi Zhitong Capsules by setting imperatorin as internal standard substance is accurate and feasible, which can be used as a useful quality control method for Compound Baizhi Zhitong Capsules.
