Simultaneous Determination of Praeruptorion A and Praeruptorion B in Tongxuan Lifei Pills by UPLC

VernacularTitle:超高液相色谱法同时测定通宣理肺丸中白花前胡甲素和白花前胡乙素
Author: Shao-Ping CHEN ¹ ; Li-Li WANG ; Yi-Jian LIANG
Author Information

1. 广西壮族自治区妇幼保健院
Keywords: praeruptorin A; praeruptorin B; Tongxuan Lifei Pills; UPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2018;25(9):76-78
CountryChina
Language:Chinese
Abstract: Objective To establish a method for content determination of praeruptorin A and praeruptorin B in Tongxuan Lifei Pills. Methods UPLC method was adopted. ACE UItra Core Super C18 (75 mm × 2.1 mm, 2.5 μm) was used with methanol-water as the mobile phase, by gradient elute. The flow rate was 0.3 mL/min; The detection wavelength was set at 321 nm; The column temperature was 40 ℃. Results Praeruptorin A and praeruptorin B showed the good linear relationship in the range of 20.48–204.8 ng (r=0.999 7) and 5.158–51.58 ng (r=1.000 0), respectively. The average recoveries were 98.2％ and 100.3％, respectively. Conclusion The method is simple, fast, accurate and reproducible, which can be used to determine the contents of praeruptorin A and praeruptorin B in Tongxuan Lifei Pills.