Effects of Jinqi Zhizhu Decoction on Gastric Motility of GK Rats with Diabetic Gastroparesis
10.3969/j.issn.1005-5304.2018.09.014
- VernacularTitle:金芪枳术汤对糖尿病胃轻瘫GK大鼠胃动力的影响
- Author:
Sheng-Fang WAN
1
;
Ya-Qi LI
;
Xiao-Li WANG
;
Chang SHU
;
Zhao-Hui WEI
;
Lei ZHANG
;
Hong-Yan WU
;
Rong-Ke LI
Author Information
1. 甘肃中医药大学
- Keywords:
diabetic gastroparesis;
Jinqi Zhizhu Decoction;
dopamine;
ACh;
5-HT;
Rho A/ROCK signaling pathway
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(9):56-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Jinqi Zhizhu Decoction (JQZZD) on gastric motility of GK rats with diabetic gastroparesis (DGP); To discuss its mechanism of action. Methods 12 out of 80 male GK rats were randomly selected as normal group, and others were induced to be DGP model by irregular feeding with high sugar and high fat diet for 8 weeks. The model rats were randomly divided into model group, positive medicine group, JQZZD low-, medium-, and high-dose groups. All rats were given continuous intragastric administration for 12 weeks. The residual rate of pigment in stomach was detected and calculated. The concentration of DA, ACh and 5-HT in plasma were detected by ELISA. The morphological changes of gastric antrum were observed by HE staining. The ultrastructure of mucous layer of gastric antrum was observed by transmission electron microscope. The protein expressions of Rho A, ROCK1 and ROCK2 were detected by Western blot. Results Compared with normal group, the residual rate of pigment in model group increased, while DA, ACh and 5-HT, relative amount of protein expressions of Rho A, ROCK1 and ROCK2 decreased (P＜0.01). Compared with model group, the residual rate of pigment in positive medicine group and JQZZD high-dose group decreased, while DA, ACh and 5-HT, relative amount of protein expressions of Rho A, ROCK1 and ROCK2 increased (P＜0.05, P＜0.01), and morphological changes and ultrastructure of mucous layer obviously improved. There was no significant differences in the residual rate of pigment, DA content and protein expressions of Rho A, ROCK1 and ROCK2 between positive medicine group and JQZZD high-dose group (P＞0.05). Compared with JQZZD low-dose group, the residual rate of pigment in JQZZD high-dose group decreased, while DA, ACh and 5-HT, relative amount of protein expressions of Rho A, ROCK1 and ROCK2 increased (P＜0.05, P＜0.01). Conclusion JQZZD may improve gastric motility, promote gastric emptying, and improve and repair damage of mucous membrane in the gastric antrum. All of these may be related to regulating protein expressions of Rho A/ROCK signal pathway.
