Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells
10. 11904/j. issn. 1002-3070. 2019. 04. 001
- VernacularTitle:红松松塔提取物多聚苯丙素-多糖复合物诱导人乳腺癌MCF-7细胞凋亡
- Author:
Weiwei AN
1
;
Yali TANG
;
Lianhua ZHAO
;
Yue YANG
;
Xinling WANG
Author Information
1. 哈尔滨医科大学肿瘤防治研究所(哈尔滨 150081
- Keywords:
Pine Cone;
Breast cancer;
MCF-7 cells;
Apoptosis;
Caspase-3;
Caspase-3 inhibitor
- From:
Practical Oncology Journal
2019;33(4):289-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective The mechanism of apoptosis induced by polyphenyl propenoid-polysaccharide complex(PPC)in hu-man breast cancer MCF-7 cells was studied. Methods MCF-7 cells were cultured with different concentrations of PPC for differ-ent time. The effects of PPC on proliferation and apoptosis were detected in MCF-7 cells by MTT assay,fluorescent staining,apopto-sis detection kit,DNA gel electrophoresis and Western blot. Results PPC induced apoptosis in MCF-7 cells. When apoptosis oc-curred,the enzyme activities of caspase-3 and-9 were increased,and the expression of pro-caspase-3 protein was decreased. Caspase-3 inhibitor(z-DEVE-fmk)could partially inhibit PPC-induced apoptosis and inhibit activation of caspase-3 precursor enzyme. Conclusion PPC induces apoptosis of MCF-7 cells by activating caspase family.
