Molecular genotyping of Giardia duodenalis in municipal waste workers in Ahvaz, southwestern Iran
- Author:
Mirzavand, S.
1
;
Kohansal, K
2
;
Beiromvand, M
1
Author Information
- Publication Type:Journal Article
- From:Tropical Biomedicine 2019;36(1):44-53
- CountryMalaysia
- Language:English
- Abstract: Giardia duodenalis is one of the most common intestinal parasites in a wide range of vertebrates, including humans and animals. It is estimated that there are approximately 200 million symptomatic giardiasis per year globally. The aim of the present study was to evaluate the prevalence and molecular diversity of G. duodenalis in municipal waste workers in Ahvaz County, southwestern Iran. This cross-sectional study was conducted among municipal waste workers aged 21 to 59 years in Ahvaz County, southwestern Iran in 2015. Stool samples collected from 400 workers were examined initially by microscopy and sucrose flotation methods, and then G. duodenalis isolates were confirmed by SSU rRNA and subsequently the genotypes were identified by triose phosphate isomerase (tpi) gene of the parasite. In total, a prevalence of 4.0% was found for G. duodenalis by microscopy and sucrose flotation methods. All microscopic-positive samples were successfully amplified at the SSU rRNA gene while tpi gene was amplified in 13 (81.25%) samples. Out of the 13 amplified isolates at tpi gene, 10 (76.9%) were typeable while the other three (23.1%) were untypeable. Assemblage A, sub-assemblage AII, was found in nine (69.2%) typeable isolates, and one (7.7%) was assemblage B, sub-assemblage BIII. The current study represents the first molecular epidemiological data on the occurrence and genotypic diversity of G. duodenalis in municipal waste workers in southwestern Iran. Assemblage A, sub-assemblage AII was the predominant assemblage of G. duodenalis. Since all infected subjects were asymptomatic, periodic examinations are recommended to prevent and control giardiasis.
- Full text:8.2019my0990.pdf
