Effects of ultrashortwave irradiation on the adhesion, proliferation and osteogenic differentiation of mesenchymal stem cells

VernacularTitle:超短波对间充质干细胞粘附增殖及成骨能力的影响
Author: Ling LIU ¹ ; Mi CHEN ; Baolan WANG
Author Information

1. 新疆医科大学第一附属医院康复医学科 830054
Keywords: Ultrashortwave irradiation; Proliferation; Cell adhesion; Mesenchymal stem cells; Osteogenic differentiation
From: Chinese Journal of Physical Medicine and Rehabilitation 2019;41(4):241-245
CountryChina
Language:Chinese
Abstract: Objective To observe the effect of ultrashortwave irradiation on the adhesion,proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (rMSCs) in rats.Methods The rMSCs were put under ultrashortwave radiation for 15 minutes every day.Early cell adhesion was detected after 1 day of the treatment.The cell morphology was observed under an inverted fluorescence microscope on the 3rd day,the cell proliferation was measured on the 1st,3rd and 7th day,while the expression of the osteogenic gene osteopontin was detected on the 8th day.Results Early cell adhesion increased 80％ (a significant increase) and more pseudo-feet with a larger range of spread were observed.After 7 days of irradiation the proliferation and the expression of osteogenic gene osteopontin had both increased significantly.Conclusion Ultrashortwave irradiation can affect the early adhesion and morphology of rMSCs and promote their proliferation and osteogenic differentiation.