Effects of total flavones of rhododendra on contractility of rat myocardial cells and its mechanism
10.19405/j.cnki.issn1000-1492.2019.02.021
- VernacularTitle:映山红花总黄酮对大鼠心肌细胞收缩性的影响及其机制
- Author:
Xiaoqing SUN
1
;
Zhiwu CHEN
Author Information
1. 安徽医科大学药理学教研室
- Keywords:
myocardial cells;
contractility;
total flavones of rhododendra;
urotensinⅡ;
Ca2 + content
- From:
Acta Universitatis Medicinalis Anhui
2019;54(2):267-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the total flavones of rhododendra(TFR) on contractility of rat myocardial cells and its possible mechanism. Methods The contraction amplitude and contraction frequency of primary cultured rat myocardial cells were observed by image analysis system. The intracellular free Ca2 + content was measured by calcium ion imaging system. Results 10 and 100 nmol /L hU Ⅱ significantly accelerated the contraction frequency of myocardial cells,and 10 nmol /L hU Ⅱ increased the contraction amplitude of myocardial cells,but 100 nmol /L hU Ⅱ reduced the contraction amplitude of myocardial cells. TFR 300 mg /L significantly slowed the contraction frequency of rat myocardial cells and increased the contraction amplitude. TFR in the range of 33. 3 ～ 300 mg /L could significantly inhibit the increase of contraction frequency,the decrease of contraction amplitude and the increase of intracellular free Ca2 + content induced by 100 nmol /L hU Ⅱ. Conclusion TFR can slow down the contraction frequency of myocardial cells and increase its contractility,which may be related to the decrease of free Ca2 + content in myocardial cells.
