Bioinformatics on vascular invasion markers in hepatocellular carcinoma via Big-Data analysis
10.3760/cma.j.issn.0254-6450.2017.04.022
- VernacularTitle:基于大数据的肝癌血管侵袭标志物分析
- Author:
Qian CHEN
1
;
Xiaoqiang QIU
Author Information
1. 广西医科大学公共卫生学院流行病学与卫生统计学教研室
- Keywords:
Hepatocellular carcinoma;
Vascular invasion;
Biomarker;
MicroRNA;
Big Data
- From:
Chinese Journal of Epidemiology
2017;38(4):522-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biomarkers in hepatocellular carcinoma and their prognostic value via GEO (Gene Expression Omnibus) and TCGA (The Cancer Genome Atlas) database.Methods Datasets of hepatocellular carcinoma were downloaded from GEO (GSE67140) and TCGA.MicroRNA in SNU423,SNU449,HepG2,Hep3B,SNU398 cell lines which had low or high invasion capabilities were investigated and verified,in 81 patients with and 91 without vascular invasion hepatocellular carcinoma.The prognostic value of these microRNAs were studied via TCGA database,obtained from 362 patients with hepatocellular carcinoma,through Kaplan-Meier and Multivariate Cox proportional hazard analysis.Target genes were analyzed by GO and KEGG.Results Expressions of hsa-mir-1180,hsa-mir-149,hsa-mir-744 and hsa-mir-940 were all up regulated in high invasion capable cell lines (SNU423,SNU449) and vascular invasion patients with hepatocellular carcinoma (logFC＞l,P＜0.05).Results from the Survival analysis showed that hsa-mir-1180 (HR=1.623,95％ CI:1.114-2.365,P=0.012),hsa-mir-149 (HR=2.400,95％ CI:1.639-3.514) and hsa-mir-940 (HR =1.704,95 ％ CI:1.188-2.443,P =0.004) were independent risk factors on the prognosis of patients with hepatocellular carcinoma (P＜0.05).The mechanism might be related to factors as immune response,focal adhesion and adherence junction signaling pathways.Conclusion With TCGA and GEO data mining,we found that hsa-mir-1180,hsa-mir-149,hsa-mir-744 and hsa-mir-940 were all highly related to the prognosis of hepatocellular carcinoma,that enabled it to be used to further study the biomarkers related to the prognosis of hepatocellular carcinoma.
