Clinical significance and expression of miR-574-5p in patients with gastric cancer
10.3760/cma.j.issn.1008-6706.2019.01.012
- VernacularTitle:微小RNA-574-5 p在胃癌组织中的表达及意义
- Author:
Fen ZHANG
1
Author Information
1. 武汉大学中南医院病理科 430071
- Keywords:
Stomach neoplasms;
Nucleic acids;
Pathology,clinical;
Neoplasm staging;
Neoplasm metastasis;
Cell differentiation;
Lymphocyte,tumor－infiltrating
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level and clinical significance of micro RNA－574－5p (miR－574－5p) in gastric carcinoma. Methods From October 2010 to October 2017,64 specimens of gastric cancer and adjacent tissues from Zhongnan Hospital were collected. Real time fluorescence quantitative technique (RT－PCR) was used to detect the expression of miR －574 －5p in specimens. Results The expression of miR－574－5p in gastric cancer tissues was (2. 37 ± 0. 69)ng/μL,and the expression of miR－574－5p in the para cancerous tissues was (0.36±0.11)ng/μL,the difference was statistically significant (t=25.32,P=0.000).The expression levels of miR－574 －5p in patients with the size of tumor size ＞5cm[(3. 04 ± 0. 74) ng/μL],TNM staging Ⅲ－Ⅳ[(3. 36 ± 0. 76)ng/μL],depth of invasion T3 －T4[(3. 29 ± 0. 72)ng/μL],low differentiation [(3.34 ±0.74)ng/μL],and distant metastasis[(3.39 ±0.78)ng/μL],were higher than those of tumor size less than 5cm[(2. 05 ± 0. 68) ng/μL],TNM stage Ⅰ－Ⅱ phase[(2. 02 ± 0. 61) ng/μL],depth of invasion T1 －T2 [(2. 07 ± 0. 64)ng/μL],middle and high differentiation[(2. 04 ± 0. 65)ng/μL]and no distant metastasis[(2. 01 ± 0. 68)ng/μL],the differences were statistically significant (t=4. 29,4. 68,4. 52,4. 62,4. 79,all P＜0. 01). Kaplan－meier analysis showed that the 5－year survival rate of patients with high expression of miR－574－5p in gastric cancer was only 16% , which was significantly lower than that in patients with low expression of miR －574 －5p (58%),and the difference was statistically significant (χ2=5. 872,P =0. 001). Conclusion miR －574 －5p expression in gastric carcinoma is high. The level of miR－574－5p expression in gastric carcinoma is related to the size of tumor,TNM stage,depth of invasion,degree of differentiation and distant metastasis. The 5－year survival rate of the patients with high expression of miR－574－5p is low.
