Clinical value of ELISA combined with colloidal gold immunochromatography in serum anti-CCP antibody detection of patients with rheumatoid arthritis
10.3760/cma.j.issn.1008-6706.2018.21.015
- VernacularTitle:酶联免疫吸附试验联合胶体金免疫层析法在类风湿关节炎患者血清抗环瓜氨酸肽抗体检测中的应用价值
- Author:
Yiqun WANG
1
;
Chao ZHANG
Author Information
1. 232000安徽省淮南,安徽理工大学医学院生化教研室;阜阳市人民医院
- Keywords:
Arthritis,rheumatoid;
Enzyme-linked immunosorbent assay;
Colloidal gold immunochromatography;
serum anti-cyclic citrullinated peptide;
Sensitivity and specificity
- From:
Chinese Journal of Primary Medicine and Pharmacy
2018;25(21):2779-2782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clincial value of ELISA combined with colloidal gold immunochromatography in the detection of anti-cyclic citrullinated peptide (CCP) antibody in patients with rheumatoid arthritis (RA).Methods From October 2014 to October 2016,120 patients with RA who admitted to Fuyang People 's Hospital were selected as RA group,and 120 healthy subjects were selected as the control group.Serum samples of patients were collected and tested for anti-CCP antibody by ELISA and ELISA combined with colloidal gold immunochromatography.Results ELISA method was lower than ELISA combined with colloidal gold immunochromatography test (x2 =3.943,P ＜ 0.05).The sensitivity,specificity,positive predictive value,negative predictive value of ELISA combined with colloidal gold immunochromatography assay were 93.33％,91.67％,84.85％,96.49％,respectively,which were higher than single ELISA method (all P ＜ 0.05).Conclusion ELISA combined with colloidal gold imnmunochromatography assay for detection of serum anti-C.CP antibody in patients with RA is effective and can obtain high diagnostic sensitivity and specificity,and it is worthy of promotion.
