Effects on G2/M Phase Cell Cycle Distribution and Aneuploidy Formation of Exposure to a 60 Hz Electromagnetic Field in Combination with Ionizing Radiation or Hydrogen Peroxide in L132 Nontumorigenic Human Lung Epithelial Cells.
10.4196/kjpp.2015.19.2.119
- Author:
Hee JIN
1
;
Hye Eun YOON
;
Jae Seon LEE
;
Jae Kyung KIM
;
Sung Ho MYUNG
;
Yun Sil LEE
Author Information
1. Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea. yslee0425@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Aneuploidy;
Extremely low frequency;
G2/M phase;
H2O2;
Ionizing radiation
- MeSH:
Aneuploidy*;
Cell Cycle*;
Cell Death;
Electromagnetic Fields*;
Epithelial Cells*;
Genomic Instability;
Humans;
Hydrogen Peroxide*;
Lung*;
Magnetic Fields;
Radiation, Ionizing*
- From:The Korean Journal of Physiology and Pharmacology
2015;19(2):119-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells were observed in the subG1 phase, which indicated the absence of apoptotic cell death. Exposure to an ELF-MF alone (1 mT or 2 mT) did not affect the percentages of G2/M-arrested cells, aneuploid cells, or the populations of cells in the subG1 phase. Moreover, when cells were exposed to a 1 mT or 2 mT ELF-MF in combination with IR (0.5 Gy) or H2O2 (0.05 mM), the ELF-MF did not further increase the percentages of G2/M-arrested cells or aneuploid cells. These results suggest that ELF-MFs alone do not induce either G2/M arrest or aneuploidy, even when administered in combination with different stressors.
