Detection of Rifampicin Resistance in Mycobacterium tuberculosis by Using Middlebrook 7H9 Broth Medium with 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride.
- Author:
Sun Min LEE
1
;
Kyung Jun KIM
;
Chulhun L CHANG
Author Information
- Publication Type:Original Article
- Keywords: Drug susceptibility test; Mycobacterium tuberculosis; Rifampin resistance
- MeSH: Genotype; Korea; Methods; Mycobacterium tuberculosis*; Mycobacterium*; Phenotype; Rifampin*; Sensitivity and Specificity; Tuberculosis
- From:Annals of Clinical Microbiology 2018;21(3):47-50
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: A simple and cost-effective method is needed for the detection of rifampicin resistance in Mycobacterium tuberculosis in resource-limited settings. We suggest a broth medium-based method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) for detection of rifampin resistance of tubercle bacilli within a reasonable time frame. METHODS: The type strain (M. tuberculosis H37Rv) and 45 cultured clinical strains of M. tuberculosis (35 rifampin-susceptible and 10 rifampin-resistant) were used. Phenotypes of rifampicin resistance were tested by the Korea Institute of Tuberculosis, and confirmed by GenoType MTBDRplus (Hain Lifescience, Germany). Susceptibility tests were performed using STC-containing OADC-enriched Middlebrook 7H9 broth (BD, USA). RESULTS: All tests were finished in 3 to 6 days. The same results were obtained with the standard and current methods for all 45 clinical isolates (100% sensitivity and specificity for resistance detection). CONCLUSION: The current method using STC is a good alternative for detecting M. tuberculosis rifampin resistance in a cost-effective and timely fashion, which is particularly important in resource-limited settings.
