Protective effect of water extract of guibi-tang against pulmonary inflammation induced by cigarette smoke and lipopolysaccharide.
- Author:
Na Rae SHIN
1
;
Tae Yang JUNG
;
Chang Seob SEO
;
So Won PARK
;
Je Won KO
;
Jong Choon KIM
;
In Sik SHIN
Author Information
- Publication Type:Original Article
- Keywords: Guibi-water extract; cigarette smoke; airway inflammation; inducible nitric oxide synthase
- MeSH: Anesthesia; Animals; Asian Continental Ancestry Group; Bronchoalveolar Lavage Fluid; Cell Count; Herbal Medicine; Humans; Inflammation; Interleukins; Lung; Memory; Mice; Nitric Oxide Synthase Type II; Peptic Ulcer; Pneumonia*; Sleep Initiation and Maintenance Disorders; Smoke*; Tobacco Products*; Tumor Necrosis Factor-alpha; Water*
- From:Laboratory Animal Research 2018;34(3):92-100
- CountryRepublic of Korea
- Language:English
- Abstract: Water extract of guibi-tang (GB), a traditional Chinese, Japanese, and Korean herbal medicine, is used to treat memory impairment, insomnia, and peptic ulcers. The aim of this study was to investigate the protective effects of GB on pulmonary inflammation induced by cigarette smoke (CS) and lipopolysaccharide (LPS). C57BL/6 mice were used to develop a pulmonary inflammation model by exposing them to CS for 1 h per day for 7 days. LPS was intranasally administered to mice under mild anesthesia on day 5. GB was administered 1 h before CS exposure at doses of 50 or 100 mg/kg for 7 days. Our results showed that GB suppressed the CS and LPS induced elevation in inflammatory cell counts in the bronchoalveolar lavage fluid (BALF), with significant reductions in protein, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 levels. Histological studies revealed that GB decreased the inflammatory cell infiltration into lung tissue caused by CS- and LPS-exposure. GB also significantly decreased the CS and LPS-induced expression of inducible nitric oxide synthase (iNOS) in the lung tissue. Taken together, GB effectively attenuated airway inflammation caused by CS and LPS. These results indicate that GB is a potential therapeutic herbal formula for pulmonary inflammatory disease.
