Inhibitory effect of the aqueous extract of a tetraploid ‘etteum’ variety in Platycodon grandiflorum on degranulation and inflammatory mediator release in RBL-2H3 cells.
10.4163/jnh.2018.51.3.208
- Author:
Jae In JUNG
1
;
Hyung Seo KIM
;
Han Kyeol JI
;
Hyun Sook LEE
;
Jae Yong LEE
;
Eun Ji KIM
Author Information
1. Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Chuncheon, Gangwon 24252, Korea. myej4@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
functional food;
Platycodon grandiflorum;
allergy;
mast cell;
inflammation mediators
- MeSH:
Animals;
Basophils;
Cytokines;
Diploidy;
Enzyme-Linked Immunosorbent Assay;
Functional Food;
Hypersensitivity;
Inflammation Mediators;
Interleukin-4;
Leukemia;
Leukotriene B4;
Mast Cells;
Medicine, East Asian Traditional;
Necrosis;
Platycodon*;
Rats;
Serum Albumin;
Tetraploidy*
- From:Journal of Nutrition and Health
2018;51(3):208-214
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Platycodon grandiflorum (a domestic diploid variety, DV-PG) has been used as a food and component of various traditional oriental medicines. Although DV-PG is known to have an anti-allergic effect, little is known about the beneficial health effects of the tetraploid ‘Etteum’ variety in the Platycodon grandiflorum (TV-PG), which is a recently developed variety. In this study, we investigated the effect of TV-PG on the rat basophilic leukemia mast cell (RBL-2H3)-mediated allergic response. METHODS: To examine the effects of TV-PG on the allergic response, RBL-2H3 cells were sensitized with dinitropheny (DNP)-immunoglobin E, treated with various concentrations of TV-PG, and challenged with DNP-human serum albumin. We estimated cell granulation by measuring the release of β-hexosaminidase and production of inflammatory mediators by ELISA. RESULTS: TV-PG had no effect on the proliferation or cytotoxicity of RBL-2H3 cells within the concentration range of 0 to 200 µg/mL. TV-PG inhibited degranulation of RBL-2H3 cells by antigen stimulation in a dose-dependent manner. TV-PG also suppressed the production of inflammatory cytokines and mediators such as interleukin-4, tumor necrosis factor-α, prostagladin E2, and leukotriene B4 in RBL-2H3 cells by antigen stimulation. CONCLUSION: These results indicate that TV-PG exhibits anti-allergic activity via inhibition of degranulation as well as suppression of inflammatory mediators and cytokine release. These findings suggest that TV-PG may have potential as a preventive and therapeutic agent for the treatment of various allergic diseases.
