Pharmacokinetic Study on Notoginsenoside R1 and Ginsenoside Rg1 in Hushen (Ⅲ) Capsules in Rats
10.3969/j.issn.1005-5304.2018.07.017
- VernacularTitle:护肾(Ⅲ)号胶囊中三七皂苷R1和人参皂苷Rg1在大鼠体内药代动力学研究
- Author:
Wei QIAN
1
;
Xing-Yue FENG
;
Xiao-Feng CHEN
;
Jian-Ping WANG
;
Zhi-Tao JIANG
;
Hui YU
Author Information
1. 南京中医药大学附属张家港医院
- Keywords:
Hushen (Ⅲ) Capsules;
UHPLC-MS/MS;
pharmacokinetics
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(7):71-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a UHPLC-MS/MS method for the determination of notoginsenoside R1 and ginsenoside Rg1 in rat plasma after oral administration of Hushen (Ⅲ) Capsules; To study its pharmacokinetics. Methods SD rats were given Hushen (Ⅲ) Capsules 2 g/kg and then the blood was collected at time point. Digoxin was used as internal standard. The drug concentrations of notoginsenoside R1 and ginsenoside Rg1 in plasma were determined by UHPLC-MS/MS. And the relative pharmacokinetic parameters were calculated by using DAS2.0 software. Results The linear relationship of notoginsenoside R1 and ginsenoside Rg1 was good (r≥0.997 5). The RSD of intra-day and inter-day precision were less than 13.1%. The results of accuracy, recovery and stability met the requirements for the biology sample analysis. For notoginsenoside R1, the pharmacokinetics parameters T1/2, Tmax, Cmaxin the plasma were (7.86±1.69)h, (4.00±1.04)h, (0.23±0.05)mg/L. For ginsenoside Rg1, the pharmacokinetics parameters T1/2, Tmax, Cmaxin plasma were (4.58±0.95)h, (6.00±0.00)h, (0.32±0.03)mg/L. Conclusion The established UHPLC-MS/MS method is sensitive, accurate and reliable, and is suitable for the pharmacokinetic study of Hushen (Ⅲ) Capsules.
