Influence of angiogenin-like protein 4 on pulmonary fibrosis

VernacularTitle:血管生成素样蛋白4(Angptl4)在肺纤维化过程中的作用
Author: Shenggang LIU ¹ ; Hongzhong YANG
Author Information

1. 410004,长沙市中心医院呼吸病诊疗中心
Keywords: Pulmonary fibrosis/PA; Angiopoietin-like 4 protein/ME; Collagen type Ⅰ/ME; Collagen type Ⅳ/ME; Rats
From: Journal of Chinese Physician 2018;20(5):712-714,719
CountryChina
Language:Chinese
Abstract: Objective To investigate the important role of angiogenin-like protein 4 (Angptl4) in pulmonary fibrosis and to provide a new therapeutic targets for pulmonary fibrosis.Methods We established the pulmonary fibrosis animal models in rat by tracheal instillation of bleomycin.Then,we detected the expression of Angptl4 through real-time polymerase chain reaction (RT-PCR) and Western Blot.Rat lung fibroblast (RLF) was transfected into Angptl4-shRNA plasmid.Then we detected the changed collagen expression in RLF cells after transfection through RT-PCR and Western blot.Results The expression of Angptl4 was up-regulated in the bleomycin-induced rat pulmonary fibrosis model.The expression of both collagen Ⅰ and collagen Ⅳ in RLF cells transfected with Angptl4-shRNA plasmids were down-regulated compared with control after TGF-β treatment.Conclusions Inhibiting the expression of Angptl4 can reduce the expression of collagen fibers in lung tissue,then delaying the progression of pulmonary fibrosis.