Effects of immunogenic HER2 on the differentiation of T-cell in mouse
10.11665/j.issn.1000 -5048.20180115
- VernacularTitle:免疫原性HER2突变体蛋白对小鼠T细胞亚群分化的影响
- Author:
Hongyu NING
1
;
Tao JIANG
;
Rui ZHANG
;
Yu HE
;
Wenbing YAO
;
Hong TIAN
Author Information
1. 中国药科大学 江苏省生物药物成药性研究重点实验室 南京210009;中国药科大学 生命科学与技术学院
- Keywords:
immunogenic amino acid;
HER2;
follicular helper T cell;
regulatory T cell;
cellular dfferentiation
- From:
Journal of China Pharmaceutical University
2018;49(1):102-108
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the effects of p-nitrophenylalanine on the differentiation of T lymphocyte,human epidermal growth factor receptor 2(HER2)mutants were constructed by incorporating p-nitrophenylalanine to the extracellular domain of HER2.Mice were immunized with HER2 mutant to analyze the effects of p-nitrophenylala-nine on the differentiation of T cell subsets.The results showed that the immunogenicity of HER2 protein was significantly enhanced by incorporation of immunogenic amino acids.HER2 mutants induced production of more anti-WT-HER2 antibody and greater titer of antibody than PBS and wild-type HER2 in the fifth week.The differ-entiation frequency of Th1 and Th2 cells showed no significant difference,but the differentiation frequency of Tfh cell was found to increase from 4％ to 8％,while the differentiation frequency of Treg cell was found to decrease from 5％ to 1％ through flowcytometry analysis.The gene expression of Bcl-6 and FOXP3 were determined by quantitative real-time PCR.The results showed that the expression of Bcl-6 mRNA increased about 2 fold,while the expression of FOXP3 mRNA could be decreased to about 50％ in HER2 mutant-immunized group.The secre-tion level of IL-21 in serum was also detected to increase from 4 to 28 pg/mL in HER2 mutant-immunized group.The above results confirm that HER2 mutants containing p-nitrophenylalanine can cause an efficient immune response by activating Tfh cell differentiation.
