Establishment of a recombinase-aided isothermal amplification technique to detect Schistosoma japonicum specific gene fragments
10.16250/j.32.1374.2018120
- VernacularTitle:重组酶介导的日本血吸虫特异性基因片段核酸等温扩增检测方法的建立
- Author:
Song ZHAO
1
;
Ting LI
;
Kun YANG
;
Wei LI
;
Jian-Feng ZHANG
;
Li-Chuan GUO
;
Yan-Hong LIU
;
Yang DAI
;
Qing-Jie YING
;
Hai-Tao YANG
Author Information
1. 国家卫生和计划生育委员会寄生虫病预防与控制技术重点实验室、江苏省寄生虫与媒介控制技术重点实验室、江苏省血吸虫病防治研究所 无锡214064
- Keywords:
Schistosoma japonicum;
Gene fragment;
Isothermal amplification of nucleic acid;
Recombinase
- From:
Chinese Journal of Schistosomiasis Control
2018;30(3):273-277,306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a novel method for the detection of Schistosoma japonicum specific gene fragments by re-combinase aided isothermal amplification(RAA).Methods The gene fragment SjG28 of S.japonicum was selected as the tar-get gene fragment to be detected,and the primers were designed according to the mechanism of RAA reaction.The reaction of isothermal amplification of S.japonicum was established and optimized.Then this method was applied to amplify and detect the specific gene fragment in the gradient diluent SjG28-recombiant plasmids and different concentrations of S.japonicum genomic DNA to estimate the sensitivity of this method.The samples were also detected by polymerase chain reaction(PCR)in parallel as control.This method was applied to detect the genomic DNA of S.mansoni,Ascaris lumbricoides,and Ancylostoma duodenale to evaluate the specificity.Results The specific gene fragment was amplified from genomic DNA of adult worms and eggs of S.japonicum by recombinase aided isothermal amplification reaction established in this study.The reaction can be completed with-in 30 minutes and the minimum detectable template was 20 copies of plasmids or 0.5 ng of genomic DNA per microliter.Other parasites'genomic DNAs,such as S.mansoni,A.lumbricoides,An.duodenale and healthy human blood genomic DNA were not able to be detected by this method.Conclusion A novel method for the detection of S.japonicum specific gene fragments by re-combinase aided isothermal amplification is established in this study,which can be carried out conveniently and rapidly with a considerable sensitivity and specificity,showing the prospect for application in the diagnosis of schistosomiasis japonica.
