Gene chip technology for detecting mycobacteria and Mycobacterium tuberculosis resistance of isoniazid and rifampin in the application of tuberculous empyema
10.3969/j.issn.1002-2694.2017.08.011
- VernacularTitle:基因芯片技术检测分枝杆菌和异烟肼、利福平耐药性在结核性脓胸诊断中的应用
- Author:
Xiu-Hua LIN
1
;
Guo-Xiang LAI
;
Yu-Yan CHEN
;
Dan ZHENG
;
Yin-Fa ZHOU
;
Xiao-Hong CHEN
Author Information
1. 福建医科大学福州总医院临床医学院呼吸与危重症医学科
- Keywords:
gene chip;
Mycobacterium;
resistance;
tuberculous empyema
- From:
Chinese Journal of Zoonoses
2017;33(8):720-723,729
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the clinical value of gene chip technology (GCT) in detecting the mycoba-cteria,isoniazid and rifampin resistance of patients diagnosed tuberculous empyema.The 182 patients who met the inclusion criteria were enrolled to this study from January 2011 to December 2015,whose pus mycobacterial species were detected by GCT and MGIT,the simultaneous and sensitivity of them were compared.Meanwhile,36 patients diagnosed tuberculous empyema were selected to detect isoniazid and rifampin resistance.The simultaneous and sensitivity of GCT were evaluated base on the standard of MGIT.The 135 patients were diagnosed by tuberculous empyema.The specificity of GCT was same to MGIT (95.7％),the the sensitivity was 48.9％ (66/135)in GCT,26.7％ in MGIT,there was significant difference between them (x2=80.5,P＜ 0.05).The sensitivity,specificity and coincidence rate of GCT in rifampin resistance were 100％,the sensitivity,specificityand coincidence rate in INH were 50.0％(1/2),97.1％(33/34) and 94.4％.Gene chip technology for detection of mycobacteria has high sensitivity and specificity,which can identify non-tuberculous mycobacteria quickly.And it can also effectively detect the resistance of Mycobacterium tuberculosis to isoniazid and rifampicin.It has important significance in early diagnosis and treatment of tuberculous empyema.
