Regulation of the apoptosis on the rat granulosa cell: effects of EGF, TGF-alpha.
- Author:
Hyun Hee JO
1
;
Seong Jin HWANG
;
Hyung Young KIM
;
Mi Ran KIM
;
Dong Jin KWON
;
Jang Heub KIM
;
Jin Hong KIM
Author Information
1. Department of Obstetrics and Gynecology, Catholic University Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Granulosa cells;
Epidermal growth factor;
Transforming growth factor;
Apoptosis
- MeSH:
Animals;
Annexin A5;
Apoptosis*;
Epidermal Growth Factor*;
Female;
Flow Cytometry;
Gene Expression;
Granulosa Cells*;
Hope;
Humans;
Ice;
Intercellular Signaling Peptides and Proteins;
Rats*;
Transforming Growth Factor alpha*;
Transforming Growth Factors
- From:Korean Journal of Obstetrics and Gynecology
2004;47(12):2439-2446
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the effect of the epidermal growth factor (EGF) and the transforming growth factor-alpha (TGF-alpha) on the apoptosis of the rat granulosa cells. METHODS: For this study, granulosa cells were isolated from female rats, and granulosa cells were cultured in the presence or absence of different autocrine/paracrine factors such as EGF and TGF-alpha. In order to investigate the apoptotic process, the number of apoptotic cells were counted using Annexin V staining via flow cytometry. In addition, the expression levels of the Bcl-2, ICE and BAX genes were checked. RESULTS: c-AMP induced apoptosis was inhibited neither by EGF nor by TGF-alpha, but FSH induced apoptosis was inhibited by EGF and TGF-alpha. Both EGF and TGF-alpha decreased the rate of apoptosis in the serum free cultured media in a time dependent manner by inducing survival gene expression such as Bcl-2 and suppressing death gene expression such as ICE and BAX. CONCLUSION: EGF and TGF-alpha can be used to decrease the apoptotic rate of the rat granulosa cells. It is hoped to conduct a similar study on the function of these growth factors in the human granulosa cells, and to use EGF and TGF-alpha for reducing the apoptosis rate of the human granulosa cells in IVF.
