van genotype and molecular typing of clinical isolates of vancomycin-resistant Enterococcus

VernacularTitle:临床分离万古霉素耐药肠球菌van基因型及分子分型
Author: Ying ZHOU ¹ ; Yang YANG ; Yan GUO ; Yonggui ZHENG ; Shi WU ; Xiaogang XU
Author Information

1. 复旦大学附属华山医院抗生素研究所
Keywords: vancomycin; resistance; gene cluster; Enterococcus faecium
From: Chinese Journal of Infection and Chemotherapy 2018;18(1):64-67
CountryChina
Language:Chinese
Abstract: Objective To determine the genotype and molecular typing of vancomycin-resistant Enterococcus (VRE).Methods Seventeen clinical isolates of VRE were collected in 2016.The strains were identified to species and confirmed by 16S rRNA sequencing.The minimum inhibitory concentration of antimicrobial agents was determined by microdilution method and agar dilution method.Multilocus sequence typing (MLST) was used for molecular typing.Results The VRE strains were confirmed as Enterococcusfaecium by 16S rRNA sequencing.All strains were resistant to vancomycin,but only 12 strains were resistant to teicoplanin.The vanA gene was identified in 13 of the 17 strains.The vanM gene was detected in 9 strains.Both vanA and vanM genes were identified in five of the 17 strains.Six MLST types were identified in the 17 strains,including ST78 (n=8),ST80 (n=4),ST555 (n=2),and one each for ST117,ST262 and ST341.Conclusions The van genotype was primarily vanA (76.5％) and vanM (52.9％) in clinical isolates of VRE.The VRE strains carrying both vanA and vanM were found for the first time.