Pigment epithelium-derived factor may play a role in apoptosis of human liver cells induced by arsenic through regulating Bcl-2 level
10.3760/cma.j.issn.2095-4255.2018.08.005
- VernacularTitle:色素上皮衍生因子通过调节Bcl-2的表达参与砷致人肝细胞线粒体凋亡
- Author:
Wei ZHANG
1
;
Xiaohui CUI
;
Liyan SUN
;
Yanhui GAO
;
Yuanyuan LI
;
Dianjun SUN
Author Information
1. 哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病防治研究所
- Keywords:
Arsenic;
Pigment epithelium-derived factor;
Bcl-2;
Bax;
Mitochondrial membrane potential
- From:
Chinese Journal of Endemiology
2018;37(8):623-626
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of pigment epithelium-derived factor (PEDF) on human liver cells in mitochondrial apoptosis induced by arsenic. Methods Human normal liver cell line (HHL-5) was exposed to NaAsO2 for 24 h, the dose was 0 (control), 2, 5, 8 and 10 μmol/L, respectively. The mitochondrial membrane potential was determined by using flow cytometry, and mRNA levels of PEDF, Bcl-2 and Bax were assayed by Real-time fluorescent quantitative PCR. Results The mitochondrial membrane potential in 10 μmol/L arsenic group (562.33 ± 31.50) was decreased markedly in comparison with that of control group (688.67 ± 47.50, P<0.01), the levels in other groups (5 μmol/L: 634.50 ± 49.03, 8 μmol/L: 655.67 ± 13.65) were not significantly changed (P > 0.05). Compared with control group [(86.69 ± 12.19)％], the mRNA levels of PEDF decreased significantly (P<0.01) when the HHL-5 cells were cultivated with 5 μmol/L [(59.35 ± 11.21)％], 8μmol/L [(58.46 ± 8.69)％] and 10μmol/L [(51.57 ± 7.58)％] arsenic, respectively, and the PEDF mRNA level in 10 μmol/L group was the lowest; but there was no significant change in PEDF mRNA level [(81.52 ± 13.93)％] when HHL-5 cell was cultivated with 2μmol/L arsenic. Similarly, mRNA level of Bcl-2 in 10 μmol/L arsenic group [(53.53 ± 21 . 49 )％] was significantly decreased compared with that of the control group [(96.48 ± 31.14)％, P < 0.05]. Whereas, Bax mRNA levels in arsenic exposure groups [(65.14 ± 32.34)％, (71.81 ± 12.61)％, (72.22 ± 28.40)％] were not significantly changed in comparison with that of control group [(84.47 ± 35.38)％, P > 0.05]. Conclusion PEDF may play a role in mitochondrial apoptosis of human liver cells induced by arsenic through regulating Bcl-2 level.
