Effect of Hypoxia on Periodontitis and the Effect of MMP-2, BGP and Inflammatory Cytokines
10.11969/j.issn.1673-548X.2018.06.041
- VernacularTitle:牙周炎大鼠MMP-2、BGP及炎性细胞因子的表达特点及低氧对于相关因子的影响
- Author:
Dandan ZHANG
1
;
Xiaohu XU
Author Information
1. 518000,深圳市龙华区中心医院口腔科
- Keywords:
Hypoxia;
Periodontitis;
Matrix metalloproteinases;
Osteocalcin;
Inflammatory cytokines
- From:
Journal of Medical Research
2018;47(6):176-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hypoxia and periodontitis on matrix metalloproteinases (MMP-2),osteocalcin (BGP) and inflammatory cytokines in periodontal tissues of rats.Methods We selected 48 healthy male SD rats.They were randomly divided into normal group (normal rats,normal oxygen periodontitis group (FED),to establish the animal model of periodontitis),hypoxia group (low pressure conditions,the combined group (FED) to establish the animal model of periodontitis + oxygen pressure) with 12 rats in each group.The rats were fed for 8 weeks in the corresponding conditions gingival index (GI),periodontal attachment loss (AL),the expression of MMP-2 anti BGP protein in periodontal tissue in the two groups was detected by Western blot assay.Immunohistochemical staining was used to detect interleukin-6 (IL-6),interleukin-18 (IL-18),tumor necrosis factor alpha (TNF-alpha),interleukin-1 beta (IL-1 beta) protein expression.Results The levels of GI,AL,IL-6,IL-18,TNF-α and IL-1β in periodontal tissues were significantly higher than those in normal group (P ＜ 0.05).The levels of GI,AL,IL-6,IL-18,TNF-α and IL-1β in the combined group were significantly higher than those in the periodontitis group and hypoxia group (P ＜0.05).The levels of MMP-2 protein in periodontal tissues of periodontitis group,hypoxia group and combined group were significantly higher than those in normal group (P ＜ 0.05).The expression of BGP protein in periodontal tissue of periodontitis group,hypoxia group and combined group (P ＜ 0.05).The levels of MMP-2 in the combined group were significantly higher than those in the periodontitis group and hypoxia group (P ＜0.05),and the level of BGP protein in the periodontal tissue of the combined group was significantly lower than that of the normal group Periodontitis group and hypoxia group (P ＜ 0.05).Conclusion The expression of MMP-2 and inflammatory factor was increased in periodontal tissues of rats with periodontitis,and the expression of BGP was significantly decreased.The hypoxic condition could promote the aggravation of periodontitis.
