Expression of astrocyte elevated gene-1 (AEG-1) in uveal melanoma and its relationship with clinical histopathology
10.13389/j.cnki.rao.2018.0026
- VernacularTitle:星形胶质细胞活化基因-1(AEG-1)在葡萄膜黑色素瘤中的表达及其与临床组织病理学的关系
- Author:
Ying MAO
1
;
Hai-Xia BAI
;
Ying CHANG
;
Fei GAO
;
Zhi-Bao ZHANG
;
Xu ZHANG
;
Bin LI
Author Information
1. 100005北京市,首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科研究所,眼科学与视觉科学北京市重点实验室
- Keywords:
uveal melanoma;
astrocyte elevated gene-1 protein;
high-risk factors;
immunohistochemistry
- From:
Recent Advances in Ophthalmology
2018;38(2):121-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expression of astrocyte elevated gene-1 (AEG-1) and its clinical pathological features in uveal melanoma.Methods Paraffm tissue specimens of 50 patients (50 eyes) with confirmed diagnosis of uveal melanoma (UM) were collected as experimental group,and samples from 11 patients (11 eyes) who underwent ocular excision due to trauma,still with largely intact uveal tissue,were selected as control group in the Department of Ophthalmology of Beijing Tongren Hospital from January 2013 to May 2017.Then the expression of AEG-1 in paraffm-embedded specimens was detected by immunohistochemical methods,and the correlation of AEG-1 expression with the clinical pathological data was analyzed.Results AEG-1 protein staining presented accurate pink or red granules,mainly located in UM nucleus,cytoplasm,cell membrane.In the experimental group,negative expression of AEG-1 was in 21 eyes (42.0％),and positive expression was in 29 eyes (58.0％),while AEG-1 were negatively expressed in the control group,and the difference in the two groups was statistically significant (P =0.000),suggesting that the expression of AEG-1 was upregulated in UM.In the experimental group,AEG-1 positive expression rate in patients with clinical pathological risk factors was significantly higher than that in patients without high risk factors (all P ＜ 0.05),and the positive expression rate of AEG-1 in UM with involvement of the ciliary body and iris neovascularization,the maximum diameter of tumor base ＞ 16 mm,tumor thickness ＞ 8 mm,tumor cell type belonging to mixed cell type and epithelioid/mixed cell type,and extra ocular tumor growth was 73.1％,73.9％,95.2％,82.4％,83.3％,74.1％ and 69.4％,respectively.However,there was no significant difference in AEG-1 expression among general clinical data including gender,eyes,age,optic disc involvement,secondary retinal detachment,epithelioid cell type and scleral duct invasion (all P ＞ 0.05).Conclusion AEG-1 is overexpressed in UM and has a significant correlation with multiple clinical risk factors,suggesting that high expression of AEG-1 may be one of the important prognostic indicators for UM.
