Expressions of SP and NK1R in eosinophil-enriched blood cells from patients with atopic dermatitis
- VernacularTitle:特应性皮炎患者嗜酸性粒细胞富集群中P物质及其受体NK1R的表达变化
- Author:
Rui-Ming YANG
1
;
Hui-Yun ZHANG
;
Wen-Jiao ZHENG
;
Jun-Ling WANG
;
Ze-Nan ZHANG
;
Shao-Heng HE
Author Information
- Keywords: atopic dermatitis(AD); eosinophil; substance P(SP); neurokinin receptor(NK1R); allergen
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2018;39(2):261-265,275
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the expressions of substance P(SP)and neurokinin receptor(NK1R)in eosinophil-enriched cells from patients with atopic dermatitis(AD)so as to elucidate their possible roles in AD. Methods Blood samples were collected from healthy controls(HCs)and AD patients,and incubated with the extracts of Artemisia pollen,dust mite,and Platanus pollen for 1 h.The expressions of SP and NK1R in eosinophil-enriched cells were detected by flow cytometry.Results Level of NK1R in eosinophil-enriched cells from AD patients increased by 41% compared with that of HCs when cultured with the medium only(P= 0.001).In addition,the expression of SP in AD patients decreased by 1.17 folds(P＜0.001),and mean fluorescence intensity (MFI)of SP+eosinophils decreased by 55%(P＜0.001).However,allergens had little effect on the expressions of SP and NK1R in eosinophil-enriched cells from AD patients and HCs.Conclusion Upregulated expression of NK1R in AD indicates that eosinophil-derived NK1R may play an important role in AD.Antagonists or blockers of NK1R might be effective preparations for AD treatment.
