Effects of RNAi-mediated CK2α gene silencing on epithelial-mesenchymal transition of laryngeal squa-mous cell
10.3969/j.issn.1006-5725.2018.08.008
- VernacularTitle:RNAi介导的CK2α基因沉默对喉癌细胞上皮间质转化过程的影响
- Author:
Fang ZHANG
1
;
Bo YANG
;
Li DU
;
Xuejun JIANG
Author Information
1. 中国医科大学附属第四医院耳鼻咽喉科 沈阳110032
- Keywords:
laryngeal squamous cell;
laryngeal carcinoma CK2α;
migration and invasion;
epitheli-al mesenchymal transition
- From:
The Journal of Practical Medicine
2018;34(8):1253-1257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of protein kinase CK2α expression on the transformation of epithelium in human laryngeal carcinoma Hep-2 cells.Methods HepG 2 cells were transfected into human laryn-geal squamous cell carcinoma cell line Hep-2 by immunization with pGCsi-H1-CK2α in vitro and divided into un-transfected group(pGCsi-H1-control)and interfering plasmid(PGCsi-H1-CK2α).The cells were transfected with G418 for 24 h and identified by Real-time PCR and Western blot.The invasion and migration ability of Hep-2 cells were detected by Transwell chamber.The patients were observed by phase contrast microscope the expression of E-cadherin,vimentin and transcription factors slug and snail were detected by Western blot. Results The expression of CK2α gene in the transfected group was significantly lower than that in the untransfected group and control plas-mid(P<0.01).Transwell experiments showed that stable knockout of CK2α prevented the migration and invasion of Hep-2 cells.Compared with untransfected and control plasmid transfection groups,the expression of E-cadherin in the interfering plasmid transfection group increased,but the expression of snail,slug and vimentin decreased. Conclusions RNAi mediated inhibition of CK2α inhibits the transformation of epithelial mesenchymal cells in la-ryngeal squamous cell carcinoma.
