Protective effects of resveratrol on human retinal pigment epithelium cells against H2O2 induced apoptosis
10.3969/j.issn.1006-5725.2018.08.006
- VernacularTitle:白藜芦醇对过氧化氢诱导人视网膜色素上皮细胞凋亡的保护作用
- Author:
Ying YANG
1
;
Zhengzheng WU
;
Yilian CHENG
;
Wei LIN
;
Chao QU
Author Information
1. 四川省医学科学院·四川省人民医院眼科 成都610072
- Keywords:
resveratrol;
cell apoptosis;
cell cycle;
retinal pigment epithelium cells
- From:
The Journal of Practical Medicine
2018;34(8):1244-1247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of resveratrol on human retinal pigment epithelium cells against H2O2-induced cell apoptosis and cell cycle. Methods Human RPE cells were divided into 3 groups ran-domly including normal control group,H2O2injury group and resveratrol protected group. H2O2injury group was treated with 200 μmol/L H2O2,and resveratrol protected group were treated with 50 mg/L resveratrol and then treat-ed with 200 μmol/L H2O2.CCK-8 was used to observe inhibitory rate of cell,and the flow cytometry was applied to detect the apoptosis and cell cycle distribution. Results CCK-8 assay showed that the inhibitory rate of cell treat-ed with resveratrol(50 mg/L)were obviously decreased,which was significantly different with H2O2injury group (P < 0.05),the apoptosis levels of cell treated with resveratrol for 2,8,24 h were significantly decreased(P <0.01).Moreover,the cell cycle distribution in the G0/G1 phases was also significantly decreased(P<0.01),the cell cycle distribution in the S phases was significantly increased(P<0.01).Conclusion Resveratrol can inhibit the cell apoptosis on human retinal pigment epithelium cells induced by H2O2,which is related to cell cycle regula-tion.
