Expression of UCH37 in cervical cancer and its effect on cell proliferation
10.3969/j.issn.1006-5725.2018.03.024
- VernacularTitle:泛素羧基末端水解酶37在宫颈癌组织中的表达及对宫颈癌细胞增殖的影响
- Author:
Xuemei YANG
1
;
Xiaodong FU
Author Information
1. 西南医科大学临床医学院 四川泸州611230;四川崇州市妇幼保健院 四川崇州611280
- Keywords:
cervical cancer;
UCH37;
proliferation;
cell apoptosis;
cell cycle
- From:
The Journal of Practical Medicine
2018;34(3):439-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of UCH37 in cervical cancer tissue,and the associa-tion of UCH37 expression and clinicopathological features of cervical cancer patients,and detect the effect of UCH37 on cell proliferation,cell apoptosis and cell cycle of HeLa cells. Methods Quantitative real-time poly-merase chain reaction(qRT-PCR)was conduct to detect the expression of UCH37 mRNA in cancer tissue and the adjacent tissue of cervical cancer patients. Spearman′s rank test was conduct to analyze the clinical relevance of UCH37 in cervical cancer. Cell count kit-8 was performed to detect the effect of UCH37 on proliferation of HeLa cells. Flow cytometry was performed to detect the effect of UCH37 on cell apoptosis and cell cycle of HeLa cells. Results qRT-PCR results revealed that UCH37 was upregulated in cervical cancer tissue compare to the adjacent tissue.Spearman rank correlation analysis showed that high UCH37 expression was significantly associated with FI-GO stage of cervical cancer patients.CCK-8 assay results showed that cell proliferation was inhibited in HeLa cells transfected with UCH37-siRNAs,and flow cytometry assays revealed that cell apoptosis was promoted in HeLa cells transfected with UCH37-siRNAs.Furthermore,compared with HeLa cells transfected with UCH37-NC,HeLa cells transfected with UCH37 siRNAs were arrested at G1 stage.Conclusion UCH37 expression is upregulated in tissue of cervical cancer,and UCH37 could promote cell proliferation of HeLa cells.UCH37 is a potential therapeu-tic target in cervical cancer.
