Effects of USP22 on cell proliferation and chemosensitivity to cisplatin in cervical cancer cells
10.3969/j.issn.1006-5725.2018.02.004
- VernacularTitle:USP22对宫颈癌细胞增殖及顺铂化疗敏感性的影响
- Author:
Chun-Yan LIU
1
;
Xiguang MAO
Author Information
1. 西南医科大学附属医院妇产科 四川泸州646000;四川省南充市中心医院妇产科 四川南充637000
- Keywords:
cervical cancer;
USP22;
proliferation;
cell apoptosis;
cisplatin
- From:
The Journal of Practical Medicine
2018;34(2):180-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of USP22 in cervical cancer cells,and the effects of USP22 on cell proliferation and chemosensitivity to cisplatin in cervical cancer cells. Methods Quantitative real-time polymerase chain reaction(qRT-PCR)was conducted to detect the expression of USP22 mRNA in cervical cancer cells.Cell count kit-8,flow cytometry,and tumorigenesis were performed to detect the effects of USP22 on the proliferation,apoptosis,cycle cycle,and chemosensitivity of cervical cancer cells to cisplatin in vitro and in vivo. Results USP22 was upregulated in cervical cancer cells compared to human immortalized epidermal cells. Cell proliferation was inhibited,cell apoptosis was promoted,cell cycle was arrested in G1 stage and chemosensi-tivity to cisplatin was enhanced in cervical cancer cells transfected with USP22-siRNAs.Furthermore,tumorigene-sis assays proved tumor growth was inhibited and chemosensitivity to cisplatin was enhanced when USP22 was silenced in HeLa cells. Conclusion USP22 expression is upregulated in cervical cancer cells,and USP22 could promote cell proliferation and inhibit chemosensitivity to cisplatin in cervical cancer cells.
