Clinical Value of Serum miRNA-21 and miRNA-126 Expression in Diagnosis of the Coronary Artery Restenosis after PCI
10.3969/j.issn.1671-7414.2018.01.015
- VernacularTitle:血清miRNA-21,miRNA-126表达在诊断PCI术后冠状动脉再狭窄的临床应用价值
- Author:
Tao CHENG
1
;
Gang HUANG
Author Information
1. 泾阳县医院检验科
- Keywords:
ACS;
PCI;
ISR;
IVUS;
miRNA-21;
miRNA-126
- From:
Journal of Modern Laboratory Medicine
2018;33(1):59-62,66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the clinical application of serum miRNA 21 and miRNA-126 in diagnosis of the coronary artery restenosis after percutaneous coronary intervention (PCI).Methods The serum and clinical data of 82 cases of acute coronary syndrome (ACS) patients treated with PCI from the Hospital of Jingyang Country and the Affiliated Hospital of Shaanxi University of Chinese Traditional Medicine (stenosis group consisted of 30 cases,non-stenosis group consisted of 52 cases),and healthy persons (control group consisted of 33 cases) were collected.The relative expression levels of serum miRNA-21 and miRNA 126 were detected using real-time qPCR technology in the comparative analysis for their changes in the groups.Intravascular ultrasound (IVUS) was used to evaluate the occurrence of restenosis after PCI.Results Serum miRNA-21 and miRNA 126 levels in the control group,the non-stenosis group and the stenosis group were,,(0.22 ± 0.03,0.43±0.08,0.92±0.17) and (0.87±0.15,0.49±0.10,0.23±0.04),respectively.Compared with the control group,the expression level of serum miRNA-21 in the stenosis group and the non stenosis group were significantly increased,while the miRNA-126 levels was significantly decreased (P=0.000),the differences were statistically significant.Compared with the non stenosis group,the level of miRNA-21 in the stenosis group was significantly increased,while the level of miRNA-126 was significantly decreased (P=0.000),the differences were statistically significant.The results of IVUS showed that the plaque area (PLA) of the patients in the stenosis group was significantly higher than that in the non stenosis group,while the minimal luman area (MLA) was significantly decreased,and the differences were statistically significant (P=0.000).There was a negative correlation between the expression of two markers in the stenosis group (r=-0.919,P＜0.01).The levels of miRNA-126 and miRNA 21 in the stenosis group were correlated with PLA and MLA (r=-0.945,0.926,P＜ 0.01) and (r=0.933,-0.917,P＜0.01).Conclusion The detection of serum miRNA-21 and miRNA-126 levels can be used in diagnosis and prediction for the coronary artary restenosis of ACS after PCI,as the gene detection index of the disease.
