Identification of Respiratory Syncytial Virus A and B Subtypesby Tube Double Nested Polymerase Chain Reaction and Gene Sequencing Technology
10.3969/j.issn.1671-7414.2018.01.011
- VernacularTitle:单管双重巢式聚合酶链反应及基因测序技术鉴别呼吸道合胞病毒A,B亚型
- Author:
Yi WU
1
;
Xian JIN
;
Chun-Hui FAN
;
Xue-Dong LU
;
Yi ZHAO
Author Information
1. 深圳市第七人民医院检验科
- Keywords:
nested PCR;
gene sequencing;
respiratory syncytial virus(RSV);
subgroup
- From:
Journal of Modern Laboratory Medicine
2018;33(1):44-48,51
- CountryChina
- Language:Chinese
-
Abstract:
Objective Objective to establish a method for identification of respiratory syncytial virus (RSV) A and B subtypes for clinical research and application.Methods Using fluorescent quantitative polymerase chain reaction (FQ-PCR) screened 50 cases of throat swab that RSV were positive in hospitalized children from 2015 to 2017.The genotyping was performed according to the nucleotide sequence of G protein coding gene,and a single tube double nested PCR primers was designed for it.A and B subgroup by sequencing to conduct comparative analysis with nucleotide sequence in the Genebank.The results were analyzed by chi-square test.Results In the 50 cases of throat swab RSV positive children,respiratory syncytial virus A and B subtype and mixed infection rates were 82.00％,14.00％ and 4.00％,respectively.The difference was statistically significant (x2=81.06,P＜0.01).The RSV fractal results were consistent with the gene sequencing results.Conclusion The eastern part of shenzhen was dominated by respiratory syncytial virus A subtype epidemic and mixed infection.Single tube double nested polymerase chain reaction (PCR) and gene sequencing technique is suitable for the identification of A and B subtypes of RSV.It is characterized by high sensitivity,specificity and high accuracy.
