Primary culture and identification of cells from human aldosterone-producing adenoma
- VernacularTitle:醛固酮瘤细胞原代培养方法的建立及细胞鉴定
- Author:
Fen WANG
1
;
An-Li TONG
;
Yu-Xiu LI
Author Information
1. 中国医学科学院 北京协和医学院 北京协和医院 内分泌科 卫生部重点实验室
- Keywords:
aldosterone-producing adenoma;
primary culture;
identification
- From:
Basic & Clinical Medicine
2018;38(6):831-834
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the primary culture of aldosterone-producing adenoma cells. Methods The tumor tissue was digested by collagen type I and cultured in complete DMEM/F12 medium. Aldosterone concentra-tion in culture medium was detected by radioimmunoassay. The expression of aldosterone synthase in the culture cells was detected by immunofluorescence. Results Aldosterone-producing adenoma cells grew adherently in a round or approximately round shape. The cells were positively immunostained for aldosterone synthase. The aldoste-rone concentration in the culture medium at the 5th culture day was (30.0±8.9)nmol/L. During the primary cul-ture,aldosterone secretion was the strongest at the first day,and decreased afterwards. It kept stable from day 4 to 11. Conclusions We successfully established the primary culture of aldosterone-producing adenoma cells, which are important for the future studying on the mechanism and function of aldosterone-producing adenoma.
