Function of RNA binding protein KSRP in type M5 acute myeloid leukemia cells
- VernacularTitle:RNA结合蛋白KSRP在急性髓系白血病(AML)M5型细胞中的功能
- Author:
Xiao-Shuang WANG
1
;
Shan YU
;
Jin-Rong HE
;
Jia YU
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 生物化学与分子生物学系医学分子生物学国家重点实验室
- Keywords:
KSRP;
RNA binding protein;
miR-129;
cell proliferation;
acute myeloid leukemia
- From:
Basic & Clinical Medicine
2018;38(6):821-826
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of RNA binding protein KSRP in multiple acute myeloid leuke-mia(AML) patients. Methods KSRP expression among AML patients and normal controls was analyzed in gene expression omnibus (GEO) datasets and the cancer genome atlas(TCGA) database. KSRP expression was inhibi-ted by lentivirus-mediated gene transduction in THP-1 cells,cell proliferation and apoptosis were analyzed. Results KSRP has lower expression in t(15;17) acute promyelocytic leukemia while has higher expression in mixed lineage leukemia(MLL) translocated acute monocytic leukemia or acute myelomonocytic leukemia. KSRP knock-down sig-nificantly promoted apoptosis (P<0.01),as well as suppressed cell proliferation (P<0.01). In addition, overex-pression of miR-129 increased cell proliferation(P<0.05). Conclusions KSRP may regulate AML-M5 cell growth through promoting miR-129 processing.
