Inhibitory effects of carboxyamidotriazole on cytokines production by peritoneal macrophages from adjuvant arthritis rats
- VernacularTitle:羧胺三唑抑制佐剂性关节炎大鼠腹腔巨噬细胞产生细胞因子
- Author:
Lei ZHU
1
;
Meng-Yuan DUAN
;
Xiao-Juan ZHANG
;
Juan LI
;
Rui JU
;
Lei GUO
;
Shan LU
;
Cai-Ying YE
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 药理系
- Keywords:
carboxyamidotriazole;
adjuvant arthritis;
macrophage;
cytokine;
nuclear factor-κB
- From:
Basic & Clinical Medicine
2018;38(6):798-802
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of carboxyamidotriazole (CAI) on cytokines production by peritoneal macrophages from adjuvant arthritis(AA) rats in vitro. Methods Freund's completed adjuvant was used to induce AA in rats.Peritoneal macrophages were prepared from asepsis and incubated with CAI(10,20,40 μmol/L).The contents of TNF-α,IL-1β and IL-6 in the culture supernatant were measured by ELISA,and mRNA expressions of TNF-α,IL-1β and IL-6 were determined by real-time quantitative PCR.NF-κB p65 DNA binding activity in the nu-clear protein was detected with TransAM kit. Results The level of TNF-α,IL-1β and IL-6 in the culture superna-tant, their intracellular mRNA expression and NF-κB p65 DNA-binding activity in peritoneal macrophages of AA rats were significantly inhibited by CAI (20 and 40 μmol/L) (P<0.05 and P<0.01).Conclusions CAI may de-crease the production of pro-inflammatory cytokines such as TNF-α,IL-1β and IL-6 through inhibiting the activa-tion of NF-κB,which is potentially associated with its anti-arthritic mechanisms.
