Phenotype change of glioma stem cells is related with the RNA binding proteins in hypoxia
- VernacularTitle:低氧微环境下胶质瘤干细胞表型的改变与RNA结合蛋白相关
- Author:
Shan-Shan LI
1
;
Pei-Shan HU
;
Wei HAN
;
Xiao-Zhong PENG
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 医学分子生物学国家重点实验室生物化学与分子生物学系 医学灵长类研究中心 神经科学中心
- Keywords:
hypoxia;
glioma stem cell;
RNA binding protein;
proliferation;
self-renewal ability
- From:
Basic & Clinical Medicine
2018;38(6):793-797
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the change of phenotype of glioma stem cells and expres-sion of RNA binding proteins in hypoxia. Methods Glioma stem cells(U87MG-SLC and GSC5) were cultured un-der hypoxia (1% O2) and normoxia(20% O2). Cell proliferation was measured by MTS assay and self-renewal ability was determined by tumorsphere formation assay. The expression of RNA binding protein and stemness mark-ers protein were examined by Western blot and statistics was carried out. Results The proliferation of glioma stem cells was inhibited and the self-renewal ability was promoted in hypoxia. Meanwhile,hypoxia significantly promoted the expression of HIF-1α and stemness markers.Under hypoxia, the expression of RNA binding protein was changed. The expression of hnRNPF, UNRIP and HuD increased. Meanwhile the expression of PCBP2 and UNR was downregulated. But,other RNA binding proteins(hnRNPK,ADAR1,PCBP1,CIRP,EBP1,eEF1A,PTBP1,PTBP2) had no significant change. Conclusions The change of phenotype of glioma stem cells in hypoxia is relat-ed with the RNA binding proteins (hnRNPF,UNRIP,HuD,PCBP2 and UNR).
