miR-210 plays a role in the transformation from mesenchymal stem cells (MSCs) into cancer-associated fibroblasts (CAFs)
- VernacularTitle:miR-210参与调控间充质干细胞向肿瘤相关成纤维细胞转化
- Author:
Hua CHEN
1
;
Jing LI
;
Chun-Hua ZHAO
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 组织工程中心
- Keywords:
miR-210;
mesenchymal stem cells;
cancer-associated fibroblasts;
breast cancer cells
- From:
Basic & Clinical Medicine
2018;38(6):780-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether miR-210 plays a crucial role in the process of inducing the transfor-mation from co-cultured adipose derived(AD)-MSCs into CAFs. Methods Co-cultured adipose derived (AD)-MSCs with breast cancer cells (BCCs) lines in vitro and detected the expression of miR-210 using RT-qPCR. The expression of CAFs characteristic markers including α-SMA and tenascin-c were measured by RT-qPCR and the ex-pression of α-SMA and FAPA were assessed by Western blot.Co-injected MCF-7 cells transfected with miR-210 in-hibitor or miR-NC with MSC at 1 : 1 ratio into the immunodeficient nude mice subcutaneously and observed tumor growth in vivo constantly. Results miR-210 was all up-regulated when a variety of breast cancer cells were co-cul-tured with MSCs (P<0.05). Inhibition of miR-210 in tumor cell could inhibit the transformation from MSCs into CAFs(P<0.05). Animal experiments further confirmed that inhibition of miR-210 in tumor cell could reduce tumor growth (P<0.05). Conclusions As an important information molecule, miR-210 mediates the transforma-tion from MSCs to CAFs in the tumor microenvironment.
