SIRT6 regulating IGF2 though recruiting mRNA binding protein IMP1 in 293T cells
- VernacularTitle:SIRT6招募mRNA结合蛋白IMP1调控IGF2在293T细胞表达
- Author:
Ting-Ting WANG
1
;
Jie WU
;
Hai-Tao DONG
;
Lai XU
;
Yi XIAO
Author Information
1. 中国医学科学院 北京协和医学院 北京协和医院中心实验室
- Keywords:
SIRT6;
IGF2;
IMP1;
tumor regulator
- From:
Basic & Clinical Medicine
2018;38(6):776-779
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the post-transcriptional regulation mechanism of insulin-like growth factor 2 (IGF2),which is an important tumor regulator,by SIRT6. Methods SIRT6 was overexpressed in 293T cells and IP was used to enrich SIRT6 and mass spectrometry(MS)was used to detect the protein that interacted with SIRT6. Western blot was used to validate the interacted protein and its acetylation/phosphorylation modification status. Results SIRT6 did not change the acetylation modification status of IMP1,but the phosphorylation status of IMP1 was elevated in the presence of SIRT6. Conclusions SIRT6 may regulate IGF2 though promoting the phosphoryla-tion of IMP1 in 293T cells.
