Study on influence of Daxx on cell cycle and chemotherapy in human ovarian cancer cells

VernacularTitle:死亡结构域相关蛋白对卵巢癌细胞周期及化疗影响的研究
Author: Weiwei LUN ¹ ; Junnai WANG
Author Information

1. 郑州大学第三附属医院妇产科 450015
Keywords: Fas-associated death domain protein; ovarian neoplasms; cell cycle; cyclineB1
From: Chongqing Medicine 2018;47(1):17-19
CountryChina
Language:Chinese
Abstract: Objective To explore the influence of down-regulating Daxx on cell cycle and chemotherapeutic drug resistance in human ovarian cancer cells.Methods SiRNA and NC negative control(NC) RNA of Daxx were constructed and divided into the NC group and silencing Daxx(siDaxx) group after transfecting to ovarian cancer cell line C13k.The doxorubicin concentration gradient of 0,0.15,0.30,0.60 μmol/L was set.The cellular cycle changes and apoptosis changes of these two groups were detected by using the flow cytometry.Western blot was used to detect the expression changes of apoptosis related protein cyclinB1 and cleavedparp.Results 0.30 μmol/L doxorubicin down-regulated Daxx to result in significant G2/M arrest(P＜0.05).Down-regulating Daxx resulted in doxorubicin resistance in C13k cells.Conclusion The effect of Daxx on ovarian cancer chemotherapy might be related to its regulation on cell cycle.