Anti-cancer effect of CT-1042 on SGC-7901 human gastric cancer cell in vitro
10.19405/j.cnki.issn1000-1492.2018.01.001
- VernacularTitle:CT-1042对SGC-7901人胃腺癌细胞抗癌效应的体外研究
- Author:
Jun YANG
1
;
Shoujun YUAN
Author Information
1. 安徽医科大学研究生学院
- Keywords:
CT-1042;
SGC-7901;
cell apoptosis;
cycle arrest;
anti-cancer
- From:
Acta Universitatis Medicinalis Anhui
2018;53(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-cancer effect of CT-1042 on human gastric cancer cells SGC-7901 in vitro. Methods The inhibitory effect of CT-1042 on proliferation of SGC-7901 cells was evaluated by MTT method, Hoechst 33342 staining was performed to observe the influence of CT-1042 on cell apoptosis morphology. The cell apoptosis rate was detected by Annexin V and PI double dye. JC-1 probe was conducted to detect the CT-1042 effects on mitochondrial membrane potential. Flow cytometry was used to determine the cell cycle distribution. Results CT-1042 could significantly inhibit the proliferation of the subject cells, and induce the nuclear fragmentation, chromatin shrinkage. CT-1042 could induce subject cells mitochondrial membrane potential decreased, lead to cell apoptosis. CT-1042 could arrest the cycle progression of the subject cells in the G2/M phase. Conclusion CT-1042 has a significant inhibitory effect on human gastric cancer cell SGC-7901 in vitro, and the mechanism underlying may contribute to induce cell apoptosis and cycle arrest.
