Protective Effects of Danlou Tablet () against Murine Myocardial Ischemia and Reperfusion Injury In Vivo.

Jian-Yong QI; Lei WANG; Dong-Sheng GU; Li-Heng GUO; Wei ZHU; Min-Zhou ZHANG

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Protective Effects of Danlou Tablet () against Murine Myocardial Ischemia and Reperfusion Injury In Vivo.

Author: Jian-Yong QI ¹ ; Lei WANG ¹ ; Dong-Sheng GU ² ; Li-Heng GUO ¹ ; Wei ZHU ³ ; Min-Zhou ZHANG ^{4
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Author Information

1. Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
2. Department of Rheumatology, The 3rd Affiliated Hospital of Southern Medical University, Guangzhou, 510630, China.
3. Network Pharmacology, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
4. Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China. minzhouzhang8@
5. com.
Publication Type:Journal Article
Keywords: Chinese medicine; Danlou Tablet; ischemia and reperfusion
MeSH: Animals; Arrhythmias, Cardiac; drug therapy; pathology; physiopathology; Body Temperature; drug effects; Cardiotonic Agents; pharmacology; therapeutic use; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; therapeutic use; Heart Rate; drug effects; Heart Ventricles; drug effects; pathology; physiopathology; Male; Mice, Inbred C57BL; Myocardial Reperfusion Injury; drug therapy; pathology; physiopathology; Risk Factors; Tablets
From: Chinese journal of integrative medicine 2018;24(8):613-620
CountryChina
Language:English
Abstract:
OBJECTIVETo observe the in vivo effect of Danlou Tablet (, DLT) on myocardial ischemia and reperfusion (I/R) injury.
METHODSDLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group (n=6), I/R group (n=8), IPC group (ischemia preconditioning, n=6) and DLT group (I/R with DLT pretreatment for 3 days, 750 mg•kg•day, n=8). The effects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo. Furthermore, the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625, 1.25, 2.5, 5 and 10 g•kg•day) were chosen in this study, and dose-response curve of DLT was obtained on these data.
RESULTSThe ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01), the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01), while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore, DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg•day.
CONCLUSIONSOur research concluded that DLT was effective in reducing I/R injury in mice, and provided experimental supports for the clinical use of DLT.