Study on ethnic medicine quantitative reference herb,Tibetan medicine fruits of Capsicum frutescens as a case.
10.19540/j.cnki.cjcmm.20180119.002
- Author:
Ke ZAN
1
;
Gan CUI
2
;
Li-Nong GUO
1
;
Shuang-Cheng MA
1
;
Jian ZHENG
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China.
2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
- Publication Type:Journal Article
- Keywords:
Capsicum frutescens;
capsaicin;
dihydrocapsaicin;
quality control;
quantitative reference herb;
specific chromatogram
- From:
China Journal of Chinese Materia Medica
2018;43(9):1764-1768
- CountryChina
- Language:Chinese
-
Abstract:
High price and difficult to get of reference substance have become obstacles to HPLC assay of ethnic medicine. A new method based on quantitative reference herb (QRH) was proposed. Specific chromatograms in fruits of Capsicum frutescens were employed to determine peak positions, and HPLC quantitative reference herb was prepared from fruits of C. frutescens. The content of capsaicin and dihydrocapsaicin in the quantitative control herb was determined by HPLC. Eleven batches of fruits of C. frutescens were analyzed with quantitative reference herb and reference substance respectively. The results showed no difference. The present method is feasible for quality control of ethnic medicines and quantitative reference herb is suitable to replace reference substances in assay.