Biosynthesis analysis of hederagenin pathway and its construction in yeast cells.
10.19540/j.cnki.cjcmm.20180307.008
- Author:
Xiao-Lin MA
1
;
Wei-Xian LI
2
;
Dong WANG
2
;
Fu-Ping LU
1
;
Zhu-Bo DAI
2
;
Xue-Li ZHANG
2
Author Information
1. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
- Publication Type:Journal Article
- Keywords:
Saccharomyces cerevisiae;
hederagenin;
oleanane type;
synthetic biology
- From:
China Journal of Chinese Materia Medica
2018;43(9):1844-1850
- CountryChina
- Language:Chinese
-
Abstract:
Hederagenin is an effective constituent of many medical plants, such as Clematidis Radix, and has a wide range of applications in anti-tumor, anti-inflammatory, antidepressant, hepatoprotective antibacterial, et al. In order to obtain the efficient production of yeast cells for hederagenin,we successfully cloned and screened out a P450 gene MdMA02 from Malus×domestica which can catalyze oleanolic acid C-23 oxidation with our developed plug and play platform. Its amino acid homology is only 32% as compared to characterized CYP72A68v2. By transforming MdMA02 to the oleanolic acid-producing strain BY-OA, a hederagenin-producing strain was constructed and hederagenin's titer could achieve 101 mg·L⁻¹ using high cell density fermentation, which was 337 times higher than in shake flasks culturing. This study provides a basis for further research on promoting the creation of oleanane-type pentacyclic triterpenoids biosynthetic pathway analysis and relative cell factories construction.
