Molecular identification of cucumber mosaic virus in woad (Isatis tinctoria) with mosaic disease in Beijing.
10.19540/j.cnki.cjcmm.20180329.006
- Author:
Jia-Li DONG
1
;
Rong WANG
1
;
Kun LIU
1
;
Yong LI
1
;
Wan-Long DING
1
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Isatis tinctoria;
RT-PCR detection;
coat protein gene;
cucumber mosaic virus;
phylogenetic trees
- From:
China Journal of Chinese Materia Medica
2018;43(11):2242-2245
- CountryChina
- Language:Chinese
-
Abstract:
To detect possible pathogenic virus(es) in woad (Isatis tinctoria) cultivated at Institute of Medicinal Plant Development in Beijing, reverse transcription(RT)-PCR was performed using total RNA of symptomatic woad leaves with primers for poty-, polero-, tobamovirus, broad bean wilt virus 2(BBWV2) and cucumber mosaic virus (CMV). A 657 bp fragment was amplified from symptomatic woad using CMV primers. Sequencing and BLAST analysis indicated that this fragment shared 99% nucleotide identity and 100% amino acid identity with CMV-Vi isolate. The isolate was named CMV-Isatis tinctorial (CMV-It). Phylogenetic analysis based on nucleotide sequences of CP genes showed that CMV-It clustered with CMV-K and belonged to subgroup I. To our knowledge, this is first identification of CMV in woad by RT-PCR and the CP gene was analyzed. This work provided data for research and control of woad mosaic disease.
