Study of isobutyrylshikonin inhibiting proliferation of colon carcinoma cells through PI3K/Akt/m-TOR pathway.
10.19540/j.cnki.cjcmm.20180130.001
- Author:
Yu ZHONG
1
;
Xue-Bao ZHENG
2
;
Kang-Rong CAI
1
;
Qiong-Lin HUANG
1
;
Hua YE
3
;
Zhe HUANG
4
;
Yong-Cun WANG
5
;
Yu-Zhen ZHU
3
Author Information
1. Analysis Center of Guangdong Medical University, Zhanjiang 524023, China.
2. Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
3. Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, China.
4. Department of Gastrointestinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
5. Oncology Center, Affiliated Hospital of Guangdong medical University, Zhanjiang 524001, China.
- Publication Type:Journal Article
- Keywords:
PI3K/AKT/m-TOR pathway;
colon carcinoma cell;
isobutyrylshikonin
- From:
China Journal of Chinese Materia Medica
2018;43(11):2358-2364
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the inhibitory effect of isobutyrylshikonin on the growth of human colon carcinoma cells and its effect on the PI3K/Akt/m-TOR pathway. MTT assay was used to detect the inhibitory effect of different concentrations (0, 6.25, 12.5, 25, 50, 100 mg·L⁻¹) of isobutyrylshikonin on the proliferation of human colon carcinoma cell HT29 at 24, 48 h. CCK-8 method was used to detect the inhibitory effect of isobutyrylshikonin on HT29, HCT116, DLD-1 and Caco-2 at 48 h. AnnexinV/propidium iodide staining was applied in detecting the apoptoticrate of HT29 cells treated with different concentrations of isobutyrylshikonin at 24 h and 48 h. Cycletest plus DNA was employed to analyze HT29 apoptosis and cell cycle after 48 h treatment with isobutyrylshikonin at different concentrations. Western blot and RT-PCR assay were used to examine the protein and mRNA expressions of PI3K, p-PI3K, Akt, p-Akt and m-TOR. The results showed that isobutyrylshikonin inhibited the proliferation of different human colon carcinoma cells, and the inhibition rate was in a dose-dependent manner. Isobutyrylshikonin induced apoptosis mainly in the early stage and blocked cells in the G₀/G₁ or G₂/M phase. Isobutyrylshikonin reduced the protein expressions of PI3K, p-PI3K, Akt, p-Akt, m-TOR and the mRNA expressions of PI3K, Akt, m-TOR in a dose-dependent manner. Isobutyrylshikonin can significantly inhibit the proliferation, induce the early apoptosis and change the cycle distribution in colon carcinoma cells.This biological effect may be correlated with the inhibition of PI3K/AKT/m-TOR pathway.
