Effects of protein kinase C and motigen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway on mRNA level of inducible nitric oxide synthase in Tca8113 cells.
- VernacularTitle:舌鳞癌细胞Tca8113中蛋白激酶C和丝裂原活化蛋白激酶激酶/细胞外调节蛋白激酶通路对诱导型一氧化氮合酶表达的影响
- Author:
Xue-Feng GAO
1
;
Hai-Bin JIAO
1
;
Chang-Cheng YE
1
;
Ying-Qun LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: cell proliferation; inducible nitric oxide synthase; motigen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway; protein kinase C
- From: West China Journal of Stomatology 2018;36(2):133-139
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the regulatory mechanism of inducible nitric oxide synthase (NOS-2) expression related to proliferation of Tca8113 cells.
METHODSRNAi mediated by short hairpin RNAs was utilized to knock down NOS-2, protein kinase C (PKC)-α, PKC-β and PKC-δ. Griess Reagent played a significant role on the detection of NO product after NOS-2 silence. The cell proliferation was determined by CCK8 method. Quantitative real-time polymerase chain reaction (q-PCR) was recruited to check the mRNA level of NOS-2, PKC-α, PKC-β and PKC-δ after treated by a variety of ways. Eventually, the measure of phosphorylation of extracellular regulated protein kinases (ERK)1/2 was performed by Western blotting in PMA-treated Tca8113 cells.
RESULTSThe cell viability of Tca8113 decreased obviously after transfected with NOS-2 siRNA (P<0.01). PKC reduced the expression level of NOS-2 mRNA (P<0.05). PKC-α, PKC-β and PKC-δ worked together to regulate the level of NOS-2 mRNA (P<0.01). Motigen-activated protein kinase kinase (MEK)/ERK signaling pathway regulated the level of NOS-2 mRNA negatively (P<0.05). PKC down regulated the level of NOS-2 mRNA through MEK/ERK signaling pathway (P<0.05).
CONCLUSIONSPKC regulates the mRNA level of NOS-2 related to proliferation through MEK/ERK signaling pathway in Tca8113 cells.
