Two new polypeptides from extract of deer bone extract.

Author: Yi REN ¹ ; Yun-Feng ZHANG ¹ ; Wen-Jing LIU ¹ ; Yun-Fang ZHAO ¹ ; Yue-Lin SONG ¹ ; Li-Qun HE ² ; Rui-Xue XIA ² ; Peng-Fei TU ¹ ; Jun LI ¹
Author Information

1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
2. Harbin Gloria Pharmaceuticals Co., Ltd., Harbin 150025, China.
Publication Type:Journal Article
Keywords: Cervus and Cucumis polypeptide injection; MALDI-TOF-MS; chemical constituent; deer bone; polypeptide
From: China Journal of Chinese Materia Medica 2018;43(12):2556-2562
CountryChina
Language:Chinese
Abstract: Two new polypeptides were isolated and purified from the extract of deer bone (constitutive part of Cucumis and Cervus polypeptide injection) by various column chromatography including C₄ 300Å and Sephadex G-50, as well as semipreparative HPLC. Their N-terminal amino acid sequences were identified by De Novo sequencing on the basis of MALDI-TOF-MS data and Explorer™ software. The N-terminal amino acid sequences of polypeptides were identified as NH₂-Gly-Pro-Val-Gly-Pro-Thr-Gly-Pro-Val-Gly-Ala-Ala-Gly-Pro-Ser-Gly-Pro-Asp (Mei18 peptide, 1) and NH₂-Ala-Gly-Pro-Ala-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Pro-Asp-Ser-Try-Asp (Mei23 peptide, 2), respectively. Mei18 and Mei 23 peptides are new polypeptides.