Rapidly identify oligosaccharides in Morinda officinalis by UPLC-Q-TOF-MSE.
10.19540/j.cnki.cjcmm.2018.0046
- Author:
Qing-Xiu HAO
1
;
Li-Ping KANG
1
;
Shou-Dong ZHU
1
;
Yi YU
2
;
Ming-Hua HU
2
;
Fang-Li MA
2
;
Jie ZHOU
3
;
Lan-Ping GUO
1
Author Information
1. The State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences， Beijng 100700, China.
2. Infinitus (China) Company Ltd., Guangzhou 510663, China.
3. University of Ji'nan, Ji'nan 250022, China.
- Publication Type:Journal Article
- Keywords:
Morinda officinalis;
UNIFI;
UPLC-Q-TOF-MSE;
identification;
oligosaccharide
- From:
China Journal of Chinese Materia Medica
2018;43(6):1201-1208
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, an approach was applied for separation and identification of oligosaccharides in Morinda officinalis How by Ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with collision energy. The separation was carried out on an ACQUITY UPLC BEH Amide C₁₈(2.1mm×100 mm，1.7 μm) with gradient elution using acetonitrile(A) and water(B) containing 0.1% ammonia as mobile phase at a flow rate of 0.2 mL·min⁻¹. The column temperature was maintained at 40 °C. The information of accurate mass and characteristic fragment ion were acquired by MSE in ESI negative mode in low and high collision energy. The chemical structures and formula of oligosaccharides were obtained and identified by the software of UNIFI and Masslynx 4.1 based on the accurate mass, fragment ions, neutral losses, mass error, reference substance, isotope information, the intensity of fragments, and retention time. A total of 19 inulin oligosaccharide structures were identified including D(+)-sucrose, 1-kestose, nystose, 1F-fructofuranosyl nystose and other inulin oligosaccharides (DP 5-18). This research provided important information about the inulin oligosaccharides in M. officinalis. The results would provide scientific basis for innovative utilization of M. officinalis.
