Difference in intestinal absorption of Wuzhuyu decoction between normal and migraine model rats.
10.19540/j.cnki.cjcmm.20180126.001
- Author:
Yan-Chuan WU
1
;
Yong-Song XU
2
;
Rui HE
2
;
Sha WU
2
;
Li GUO
2
;
Mu-Xin GONG
2
;
Zhi-Min WANG
3
Author Information
1. Central Laboratory, Xuanwu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Beijing 100053, China.
2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Wuzhuyu decoction;
intestinal absorption;
migraine model;
optimized sample;
single-pass intestinal perfusion
- From:
China Journal of Chinese Materia Medica
2018;43(8):1682-1691
- CountryChina
- Language:Chinese
-
Abstract:
To compare the intestinal absorption of Wuzhuyu decoction(WZYD) between normal rats and migraine model rats, and investigate the optimized WZYD from aspect of absorption. The rat single pass intestinal perfusion test(SPIP) was adopted for optimized sample and un-optimized sample in normal and migraine model rats induced by nitroglycerin and reserpine. The contents of 8 ingredients were determined by high performance liquid chromatography(HPLC), and 4 absorption parameters for each ingredient were calculated and compared: unit area absorption(Mper area), absorption rate constant(Ka), apparent coefficient(Papp) and relative absorption rate(RA). The results showed that there was a great difference between normal rats and model rats in the intestinal absorption of the same WZYD. As compared with normal rats, the absorption parameters of most ingredients in optimized sample were increased in migraine model rats induced by nitroglycerin; Similar phenomena were also found in migraine model rats induced by reserpine. However, the absorption parameters of most ingredients were decreased in un-optimized sample. Therefore, pathological model rats shall be used for effective ingredient recognition based on the correlation between intestinal absorption spectra and pharmacological effects. As compared with the un-optimized samples, the absorption of effective ingredients was faster, easier and more adequate in the optimized samples, revealing their mechanism on better efficacy from the aspect of absorption.
